Research Professional II - Sarver Heart Center

This Research Professional II - Sarver Heart Center position is with University of Arizona in Tucson, AZ. Research Colony Management Manage investigator-derived mouse lines for the PI’s research program. Plan and organize breeding strategies to produce the genotypes and cohort sizes required for planned experiments, adjusting them based on colony data in consultation with the PI. Perform, optimize, and troubleshoot genotyping assays (PCR and enzymatic methods). Interpret sequencing results to verify alleles and detect genetic drift or contamination. Maintain genotype and pedigree records for all colony animals, and prepare colony reports to support experimental planning. Administer treatments and collect biological samples in accordance with approved IACUC protocols. Experimental Research Design components of in vivo and in vitro experiments within the PI’s overall research design, and conduct studies that support project objectives. Perform molecular and biochemical analyses, including enzymatic assays, Western blotting, mitochondrial function assessment, and metabolite quantification. Analyze and interpret experimental data and recommend solutions to methodological problems. Recommend and implement improvements to laboratory procedures and protocols. Research Program Support and Collaboration Present findings at lab meetings and participate in the writing and research for publications and presentations. Draft and update standard operating procedures for review by research leadership and maintain laboratory records. Mentor students and support staff in laboratory techniques. Surgical and Imaging Support Serve as the trained backup to the lead cardiac microsurgeon for murine myocardial infarction and ischemia/reperfusion procedures. This includes anesthesia, intubation, aseptic setup, and intraoperative support. Provide post-operative monitoring and recognize surgical complications. Acquire and analyze echocardiography data (ejection fraction, fractional shortening, and ventricular dimensions). Maintain surgical and imaging documentation and keep cardiac studies running when the lead surgeon is unavailable. Knowledge, Skills, and Abilities (KSAs): Ability to work with animal models