This Research Technician III position is with University of Arizona in Tucson, AZ.
Independently manage multiple mouse colonies: plan breeding crosses to meet study needs; monitor breeding performance, births, weaning, health and aging cohorts; identify and track animals; collect samples and perform PCR genotyping; maintain accurate colony databases and coordinate animal availability with research staff. Troubleshoot breeding and colony-management problems and communicate issues promptly. Perform and coordinate animal experiments under approved IACUC protocols, including handling and restraint, drug preparation and administration by approved injection routes, anesthesia and perioperative monitoring, approved survival or nonsurvival surgical procedures, and behavioral testing. Monitor animal welfare, document procedures, and recognize and escalate complications. Collect tissues and biological specimens at defined experimental endpoints using approved euthanasia and dissection methods. Perform perfusion and brain/spinal cord and peripheral tissue dissections as required; process, label, preserve, inventory, and transfer specimens for histology, molecular assays and other analyses. Maintain sample traceability and accurate experimental records. Coordinate day-to-day animal study logistics: schedule breeding, aging cohorts, treatments, surgeries and tissue collections; prepare supplies and maintain equipment and inventories; record observations and experimental results; troubleshoot technical workflows; and work with veterinary and animal-care personnel to maintain protocol compliance and research continuity. Train and guide students and junior laboratory personnel in mouse handling, breeding records, genotyping, injections, specimen collection, aseptic technique and documentation, as authorized. Reinforce laboratory standard operating procedures, animal welfare practices and required training records; identify opportunities to improve reproducibility and workflow efficiency. Train and guide students and junior laboratory personnel in mouse handling, breeding records, genotyping, injections, specimen collection, aseptic technique and documentation, as authorized. Reinforce laboratory standard operating procedures, animal welfare practices and required training records; identify opportunities to improve reproducibility and workflow efficiency. Knowledge, Skills and Abilities: Ability to provide advanced mouse breeding and colony recordkeeping; PCR genotyping; animal handling, injections, aseptic procedures and tissue collection. Ability to provide careful scheduling and sample tracking; independent technical troubleshooting; clear communication; animal welfare, IACUC protocol compliance and documentation. Ability to guide and train junior staff.